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ContextMap 2.0

Sequencing of RNA (RNA-seq) using next generation sequencing has become the standard approach for profiling the transcriptomic state of a cell. This requires mapping of the sequencing reads to determine their transcriptomic origin.
Recently, we developed a context-based mapping approach, ContextMap, which determines the most likely origin of a read by evaluating the context of the read in the form of alignments of other reads to the same genomic region. In the original implementation, the focus was on improving initial mappings provided by other mapping tools.

Here, we present ContextMap 2.0, an extension of the original ContextMap method, which can also be used as a standalone tool without relying on initial mappings by other tools. We show that it yields highly accurate read mappings and is very robust against sequencing errors. The design of ContextMap 2.0 allows for massively parallelized data processing, resulting in reasonable running times despite the higher complexity of the context-based approach.

Publications
2012
Papers
Thomas Bonfert, Gergely Csaba, Ralf Zimmer, Caroline C. Friedel. A context-based approach to identify the most likely mapping for RNA-seq experiments. BMC Bioinformatics, vol 13(Suppl 6), pp. S9, 2012.
2013
Papers
Thomas Bonfert, Gergely Csaba, Ralf Zimmer, Caroline C. Friedel. Mining RNA-Seq Data for Infections and Contaminations. PloS one, vol 8, pp. e73071, Sep 2013.
Downloads
Current release version: 2.2.1

Latest changes:
  • ContextMap can be used to mine for infections and contaminations (see the updated manual for details)
  • Support of paired-end data
  • Support of strand-specific data
  • Runtime and memory usage improvements at the global resolution step
ContextMap v.2.2.1 Unix contextmap_v2.2.1_Unix.tar.gz
ContextMap v.2.2.1 MacOS X contextmap_v2.2.1_MacOS_X.tar.gz
ContextMap v.2.2.1 Source contextmap_v2.2.1_Source.tar.gz
Manual Manual
In silico data sets in_silico_sets.tar.gz
Supplementary Material Supplementary Material
Upcoming
  • The upcoming ContextMap release will work with unmodified versions of Bowtie, Bowtie2 and BWA
  • Mapping speed improvements by factor 3 or more
  • Support of variable read lengths
  • Detection of reads spanning over an arbitrary number of exons


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