@article {bioinflmu-255,
	title = {{Microarray analyses of transdifferentiated mesenchymal stem cells}},
	journal = {Journal of Cellular Biochemistry},
	volume = {103},
	number = {2},
	year = {2008},
	pages = {413-433},
	abstract = {The molecular events associated with the age-related gain of fatty

tissue in human bone marrow are still largely unknown. Besides enhanced

adipogenic differentiation of mesenchymal stem cells (MSCs), transdifferentiation

of osteoblast progenitors may contribute to bone-related diseases like

osteopenia. Transdifferentiation of MSC-derived osteoblast progenitors into

adipocytes and vice versa has previously been proven feasible in our cell culture

system. Here, we focus on mRNA species that are regulated during

transdifferentiation and represent possible control factors for the initiation of

transdifferentiation. Microarray analyses comparing transdifferentiated cells

with normally differentiated cells exhibited large numbers of reproducibly

regulated genes for both, adipogenic and osteogenic transdifferentiation. To

evaluate the relevance of individual genes, we designed a scoring scheme to rank

genes according to reproducibility, regulation level, and reciprocity between the

different transdifferentiation directions. Thereby, members of several signaling

pathways like FGF, IGF, and Wnt signaling showed explicitly differential

expression patterns. Additional bioinformatic analysis of microarray analyses

allowed us to identify potential key factors associated with transdifferentiation

of adipocytes and osteoblasts, respectively. Fibroblast growth factor 1 (FGF1)

was scored as one of several lead candidate gene products to modulate the

transdifferentiation process and is shown here to exert inhibitory effects on

adipogenic commitment and differentiation.},
	keywords = {expressionlab@lmu},
	doi = {10.1002/jcb.21415},
	author = {K{\"u}ffner, R and Tatjana Schilling and Ralf Zimmer and Ludger Klein-Hitpass and Franz Jakob and Norbert Sch{\"u}tze}
}