@Article{LotzHavla2021,
  author          = {Lotz-Havla, Amelie S. and Woidy, Mathias and Guder, Philipp and Friedel, Caroline C. and Klingbeil, Julian M. and Bulau, Ana-Maria and Schultze, Anja and Dahmen, Ilona and Noll-Puchta, Heidi and Kemp, Stephan and Erdmann, Ralf and Zimmer, Ralf and Muntau, Ania C. and Gersting, Sören W.},
  journal         = {Journal of proteome research},
  title           = {iBRET Screen of the ABCD1 Peroxisomal Network and Mutation-Induced Network Perturbations.},
  year            = {2021},
  issn            = {1535-3907},
  month           = sep,
  pages           = {4366--4380},
  volume          = {20},
  abstract        = {Mapping the network of proteins provides a powerful means to investigate the function of disease genes and to unravel the molecular basis of phenotypes. We present an automated informatics-aided and bioluminescence resonance energy transfer-based approach (iBRET) enabling high-confidence detection of protein-protein interactions in living mammalian cells. A screen of the ABCD1 protein, which is affected in X-linked adrenoleukodystrophy (X-ALD), against an organelle library of peroxisomal proteins demonstrated applicability of iBRET for large-scale experiments. We identified novel protein-protein interactions for ABCD1 (with ALDH3A2, DAO, ECI2, FAR1, PEX10, PEX13, PEX5, PXMP2, and PIPOX), mapped its position within the peroxisomal protein-protein interaction network, and determined that pathogenic missense variants in   alter the interaction with selected binding partners. These findings provide mechanistic insights into pathophysiology of X-ALD and may foster the identification of new disease modifiers.},
  chemicals       = {ATP Binding Cassette Transporter, Subfamily D, Member 1, ATP-Binding Cassette Transporters, Fatty Acids},
  citation-subset = {IM},
  completed       = {2021-10-28},
  country         = {United States},
  doi             = {10.1021/acs.jproteome.1c00330},
  issn-linking    = {1535-3893},
  issue           = {9},
  keywords        = {ATP Binding Cassette Transporter, Subfamily D, Member 1, genetics; ATP-Binding Cassette Transporters, genetics, metabolism; Animals; Energy Transfer; Fatty Acids; Informatics; Mutation; ABCD1; BRET; FAR1; X-ALD; bioluminescence resonance energy transfer; fatty acids; interactome; lipid droplets; living cells; protein?protein interaction; screening},
  nlm-id          = {101128775},
  owner           = {NLM},
  pmid            = {34383492},
  pubmodel        = {Print-Electronic},
  pubstate        = {ppublish},
  revised         = {2021-10-28},
}