@article {bioinflmu-1158,
	title = {{Ultrashort and progressive 4sU-tagging reveals key characteristics of RNA processing at nucleotide resolution.}},
	journal = {Genome research},
	volume = {22},
	year = {2012},
	month = {2012 Oct},
	pages = {2031-42},
	abstract = {RNA synthesis and decay rates determine the steady-state levels of cellular RNAs. Metabolic tagging of newly transcribed RNA by 4-thiouridine (4sU) can reveal the relative contributions of RNA synthesis and decay rates. The kinetics of RNA processing, however, had so far remained unresolved. Here, we show that ultrashort 4sU-tagging not only provides snapshot pictures of eukaryotic gene expression but, when combined with progressive 4sU-tagging and RNA-seq, reveals global RNA processing kinetics at nucleotide resolution. Using this method, we identified classes of rapidly and slowly spliced/degraded introns. Interestingly, each class of splicing kinetics was characterized by a distinct association with intron length, gene length, and splice site strength. For a large group of introns, we also observed long lasting retention in the primary transcript, but efficient secondary splicing or degradation at later time points. Finally, we show that processing of most, but not all small nucleolar (sno)RNA-containing introns is remarkably inefficient with the majority of introns being spliced and degraded rather than processed into mature snoRNAs. In summary, our study yields unparalleled insights into the kinetics of RNA processing and provides the tools to study molecular mechanisms of RNA processing and their contribution to the regulation of gene expression.},
	keywords = {Alternative Splicing, B-Lymphocytes, Cell Line, Exons, Humans, Introns, Kinetics, RNA, RNA Precursors, RNA Splice Sites, RNA Splicing, RNA Stability, Thiouridine, Transcription, Genetic},
	doi = {10.1101/gr.131847.111},
	author = {Lukas Windhager and Thomas Bonfert and Kaspar Burger and Zsolt Ruzsics and Stefan Krebs and Stefanie Kaufmann and Georg Malterer and Anne L{\textquoteright}Hernault and Markus Schilhabel and Stefan Schreiber and Philip Rosenstiel and Ralf Zimmer and Dirk Eick and Caroline C. Friedel and Lars D{\"o}lken}
}