@article {bioinflmu-405,
	title = {{Comparison of the chondrosarcoma cell line SW1353 with primary human adult articular chondrocytes with regard to their gene expression profile and reactivity to IL-1beta}},
	journal = {Osteoarthritis Cartilage},
	volume = {13},
	number = {8},
	year = {2005},
	pages = {697-708},
	abstract = {OBJECTIVE: In this study, the human chondrosarcoma cell line SW1353
was investigated by gene expression analysis in order to validate it as an in
vitro model for primary human (adult articular) chondrocytes (PHCs). METHODS:
PHCs and SW1353 cells were cultured as high density monolayer cultures with and
without 1ng/ml interleukin-1beta (IL-1beta). RNA was isolated and assayed using a
custom-made oligonucleotide microarray representing 312 chondrocyte-relevant
genes. The expression levels of selected genes were confirmed by real-time
polymerase chain reaction and the gene expression profiles of the two cell types,
both with and without IL-1beta treatment, were compared. RESULTS: Overall, gene
expression profiling showed only very limited similarities between SW1353 cells
and PHCs at the transcriptional level. Similarities were predominantly seen with
respect to catabolic effects after IL-1beta treatment. In both cell systems
matrix metalloproteinase-1 (MMP-1), MMP-3 and MMP-13 were strongly induced by
IL-1beta, without significant induction of MMP-2. IL-6 was also found to be
up-regulated by IL-1beta in both cellular models. On the other hand,
intercellular mediators such as leukemia inhibitory factor (LIF) and bone
morphogenetic protein-2 (BMP-2) were not induced by IL-1beta in SW1353 cells, but
significantly up-regulated in PHCs. Bioinformatical analysis identified nuclear
factor kappa-B (NFkappaB) as a common transcriptional regulator of IL-1beta
induced genes in both SW1353 cells and PHCs, whereas other transcription factors
were only found to be relevant for individual cell systems. CONCLUSION: Our data
characterize SW1353 cells as a cell line with only a very limited potential to
mimic PHCs, though SW1353 cells can be of value to study the induction of
protease expression within cells, a phenomenon also seen in chondrocytes.},
	keywords = {cell line SW1353},
	doi = {10.1016/j.joca.2005.04.004},
	author = {M. Gebauer and Joachim Saas and Florian Sohler and Jochen Haag and S. Soder and M. Pieper and Eckart Bartnik and J. Beninga and Ralf Zimmer and Thomas Aigner}
}