@article {bioinflmu-515,
	title = {{Reconstruction, modeling \& analysis of Halobacterium salinarum R-1metabolism}},
	journal = {Mol Biosyst},
	volume = {4},
	number = {2},
	year = {2008},
	pages = {148-59},
	abstract = {We present a genome-scale metabolic reconstruction for the extreme

halophile Halobacterium salinarum. The reconstruction represents a summary of the

knowledge regarding the organism{\textquoteright}s metabolism, and has already led to new

research directions and improved the existing annotation. We used the network for

computational analysis and studied the aerobic growth of the organism using

dynamic simulations in media with 15 available carbon and energy sources.

Simulations resulted in predictions for the internal fluxes, which describe at

the molecular level how the organism lives and grows. We found numerous

indications that cells maximized energy production even at the cost of longer

term concerns such as growth prospects. Simulations showed a very low carbon

incorporation rate of only approximately 15\%. All of the supplied nutrients were

simultaneously degraded, unexpectedly including five which are essential. These

initially surprising behaviors are likely adaptations of the organism to its

natural environment where growth occurs in blooms. In addition, we also examined

specific aspects of metabolism, including how each of the supplied carbon and

energy sources is utilized. Finally, we investigated the consequences of the

model assumptions and the network structure on the quality of the flux

predictions.},
	doi = {10.1039/b715203e},
	author = {Orland Gonzalez and S. Gronau and M. Falb and F. Pfeiffer and E. Mendoza and Ralf Zimmer and D. Oesterhelt}
}